|
Voges–Proskauer or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown color indicates a negative result.〔MacFaddin, J. F. 1980. Biochemical Tests for Identification of Medical Bacteria, 2nd ed. Williams and Wilkins, Baltimore〕 The test depends on the digestion of glucose to acetylmethylcarbinol. If glucose is being broken down, it will react with alpha-naphthol (VP reagent #1) and potassium hydroxide (VP reagent #2) to form a red color. Alpha-naphthol and potassium hydroxide are chemicals that detect acetoin. Procedure: First, add the alpha-naphthol; then, add the potassium hydroxide. A reversal in the order of the reagents being added may result in a weak-positive or false-negative reaction. VP is one of the four tests of the IMViC series, which tests for evidence of an enteric bacterium. The other three tests include: the indole test (), the methyl red test (), and the citrate test ().〔Bachoon, Dave S., and Wendy A. Dustman. Microbiology Laboratory Manual. Ed. Michael Stranz. Mason, OH: Cengage Learning, 2008. Exercise 15, "Normal Flora of the Intestinal Tract" Print.〕 VP positive organisms include ''Enterobacter'', ''Klebsiella'', ''Serratia marcescens'', ''Hafnia alvei'', ''Vibrio eltor'', and ''Vibrio alginolyticus''.〔Bergey's Manual of Systematic Bacteriology, Vol. 1. Baltimore, Williams and Wilkins, 1984.〕 VP negative organisms include ''Citrobacter'' sp., ''Shigella'', ''Yersinia'', ''Edwardsiella'', ''Salmonella'', ''Vibrio furnissii'', ''Vibrio fluvialis'', ''Vibrio vulnificus'', and ''Vibrio parahaemolyticus''.〔 ==History== The reaction was developed by Daniel Wilhelm Otto Voges and Bernhard Proskauer—German bacteriologists in 1898 at the Institute for Infectious Diseases. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Voges–Proskauer test」の詳細全文を読む スポンサード リンク
|